Seven people were arrested as officers broke up the Occupy Charlotte tent city, across the street from police headquarters.
Those arrested Monday faced misdemeanor charges of resist, obstruct and delay. The action took about an hour.
Some activists were angry and shouted: "Evict us and we'll multiply" as police officers searched more than 20 remaining tents.
The move by Charlotte-Mecklenberg police came one week after a city ordinance was adopted restricting political demonstrations ahead of this year's Democratic National Convention in Charlotte.
The new rules approved by Charlotte City Council give police more power to stop and search people when the convention comes to town in September.
But a major part of the ordinance outlaws camping overnight on city property.
Police Chief Rodney Monroe had warned Occupy Charlotte that the city would take action Monday -- the day the ordinance took effect.